منابع مشابه
Chymotryptic digestion of Tetrahymena 22S dynein. I. Decomposition of three-headed 22S dynein to one- and two-headed particles
Molecular composition of Tetrahymena ciliary dynein has been examined by electron microscopy and gel electrophoresis. SDS-urea gel electrophoresis revealed that Tetrahymena 22S dynein contains three (A alpha, A beta, and A gamma) heavy chains whereas 14S dynein contains only one. The molecular masses of 22S and 14S dynein heavy chains were estimated to be approximately 490 and 460 kD, respectiv...
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A cytoplasmic dynein is a microtubule-based motor protein involved in diverse cellular functions, such as organelle transport and chromosome segregation. The dynein has two ring-shaped heads that contain six repeats of the AAA domain responsible for ATP hydrolysis. It has been proposed that the ATPase-dependent swing of a stalk and a stem emerging from each of the heads generates the power stro...
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Most synthetic inhibitors of peptidases have been targeted to the active site for inhibiting catalysis through reversible competition with the substrate or by covalent modification of catalytic groups. Cathepsin B is unique among the cysteine peptidase for the presence of a flexible segment, known as the occluding loop, which can block the primed subsites of the substrate binding cleft. With th...
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The immune response is initiated when an antigen is recognized by specific lymphocytes. Originally, ‘antigen’ was viewed as a simple entity that bound directly to lymphocyte antigen receptors: the B-cell antigen receptor complex that includes membrane-bound immunoglobulin, or the T-cell antigen receptor. It has been clear for a number of years, however, that conventional T cells recognize not f...
متن کاملMicrotubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia
Tetrahymena cilia contain a three-headed 22S (outer arm) dynein and a single-headed 14S dynein. In this study, we have employed an in vitro assay of microtubule translocation along dynein-coated glass surfaces to characterize the motile properties of 14S dynein, 22S dynein, and proteolytic fragments of 22S dynein. Microtubule translocation produced by intact 22S dynein and 14S dynein differ in ...
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1981
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.2.73